Viral vectors laboratory (LVV)

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About the facility

The Viral Vectors Laboratory (LVV) specializes in the production of recombinant viruses for research and biotechnology applications. The facility operates in a level 2 biosafety environment and is accredited by the National Technical Commission on Biosafety (CTNBio). LVV supports the production of non-replicating recombinant virus preparations, including murine retroviral vectors and human lentiviral vectors derived from HIV-1.

These systems are widely used for gene expression, silencing by RNA interference and generation of modified cell lineages. The facility contributes to the advancement of research aimed at understanding intracellular mechanisms and the development of gene and cell therapies applied to human health.

How to submit research proposals

Proposals must be submitted through SAU Online. In case of any doubt during the proposal submission, contact the CNPEM Users Office by email at edu@cnpem.br or speak to those responsible for the facility by writing to lvv@lnbio.cnpem.br.

SUBMIT YOUR PROPOSAL

SEE THE FAQ

Equipment

The LVV is installed in a culture room dedicated to the production of viral preparations, with the following equipment:

  • Class II safety cabinets;
  • CO₂ incubators for cell lineage maintenance;
  • Inverted microscope;
  • Preparative centrifuges;
  • Ultracentrifuge for particle concentration;
  • –80º C Biofreezer for production preservation;
  • Novocyte 2000 flow cytometer.

Main competencies and techniques

  • Production of murine retroviral vectors with ecotropic, amphotropic, 10A1, RD114 or VSV-G envelopes;
  • Production of lentiviral vectors derived from human HIV-1 with the same envelopes;
  • Customized productions with envelopes provided by the applicant, subject to technical feasibility.

meet our team

Marcio C. Bajgelman

Coordinator

Viral vectors laboratory (LVV)
lvv@lnbio.cnpem.br

Daniela S. Mizobuti

Specialist

Viral vectors laboratory (LVV)
lvv@lnbio.cnpem.br