Advanced Omics Laboratory

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The Advanced Omics Laboratory (LOA) is a multidisciplinary space dedicated to sample processing for different omics applications, integrating flow cytometry technologies, high-precision cell sorting and single-cell analysis. Its infrastructure permits phenotypic and morphological characterization of cells, separation of specific subpopulations with high purity, and refined preparation of samples for genomic, transcriptomic, proteomic and other approaches. With cutting-edge equipment and optimized protocols, the laboratory offers complete support for research projects that require high cellular resolution and quality in the preparation of biological materials.

How to submit research proposals

LOA currently operates on a continuous basis for internal CNPEM users only.

Equipment

  • FACSDiscover S8 Cell Sorter flow cytometer (BD)
  • FACSCanto II flow cytometer (BD)
  • Celigo imaging cytometer (Revvity)
  • CellenONE X1 single cell separation platform (Cellenion)
  • Chromium iX single cell separation platform (10X Genomics)
  • Oroboros O2k Fluorespirometer (Oroboros Instruments)
  • Seahorse XFe24 Fluorespirometer (Agillent)
  • Nanosight NS300 nanoparticle analyzer (Malvern Panalytical)

Main competencies and techniques

  • Immunophenotyping
  • Intracellular cytokine dosage and secretion (using cytometric bead array, CBA)
  • Cell sorting
  • Studies on cell signaling and internalization, analysis of cell proliferation and migration
  • Studies with stem cells and organoids
  • Isolation and preparation of cells for single-cell RNA-seq
  • Isolation of a single cell for cloning in culture
  • Isolation and preparation of cell samples for single-cell proteomics
  • Studies on mitochondrial function
  • Quantification and characterization of nanoparticles in liquid suspension

meet our team

Juliana Ferreira de Oliveira

Lead Researcher (Interim)

loa@lnbio.cnpem.br

Tatiane De Rossi Mazo

Specialist

Elaine Cristina Cardoso

Specialist

All groups in advanced methodologies in biology