Facility’s utilization by academic users is always free of charge, and proposal submissions are carried out online
Between December 10th, 2024 and January 15th, 2025, the Brazilian Center for Research in Energy and Materials (CNPEM) will receive research proposals from the external academic community for use of the Protein Purification Laboratory (LPP). The proposals will be carried out between February and June 2025.
The Protein Purification Laboratory (LPP) from the Brazilian Biosciences National Laboratory (LNBio) offers a complete structure and inputs for E. coli expression and recombinant protein purification. The facility has a set of FLPC (Fast protein liquid chromatography) chromatographs for protein separation, with availability of affinity, ion exchange, hydrophobic interaction and size exclusion chromatographic columns.
The facility’s team offers scientific support in optimizing methods, choosing columns and recommending buffer solutions based on the target molecule to be purified, besides the equipment usage training and complete support in carrying out the separation processes. The LPP provides a supportive and learning environment, aiming to obtain pure and stable protein samples, suitable for use in different subsequent functional and structural studies.
It’s worth of notice that the user must take part in person of the whole experimental process, in addition to providing the expression vector for the protein of interest and the expression and purification protocol’s reference, from the literature or previously tested.
The proposal’s selection criteria includes proposed experimental protocol technical feasibility, considering the equipment, inputs and techniques available at the LPP capabilities. Access the facility’s offered portfolio.
Proposal submission will be carried out exclusively through the SAU online system. Users from outside the State of São Paulo may request travel assistance – find out more on the CNPEM Users Office (EdU) assistance page.
Key dates
Submission start: December 10th, 2024
Submission deadline: January 15th, 2025
Selected proposals disclosure: January 30th, 2025
Execution phase: February to June 2025
About CNPEM
The Brazilian Center for Research in Energy and Materials (CNPEM) is home to a state-of-the-art, multi-user and multidisciplinary scientific environment and works on different fronts within the Brazilian National System for Science, Technology and Innovation. A social organization overseen by the Ministry of Science, Technology and Innovation (MCTI), CNPEM is driven by research that impacts the areas of health, energy, renewable materials, and sustainability. It is responsible for Sirius, the largest assembly of scientific equipment constructed in the country, and is currently constructing Project Orion, a laboratory complex for advanced pathogen research. Highly specialized science and engineering teams, sophisticated infrastructure open to the scientific community, strategic lines of investigation, innovative projects involving the productive sector, and training for researchers and students are the pillars of this institution that is unique in Brazil and able to serve as a bridge between knowledge and innovation. It is responsible for operating the Brazilian Synchrotron Light (LNLS), Biosciences (LNBio), Nanotechnology (LNNano), and Biorenewables (LNBR) National Laboratories, as well as the Ilum School of Science, which offers a bachelor’s degree program in science and technology with support from the Ministry of Education (MEC).
