Seminar: Structural and functional insights into the multilayered mechanisms involved in intracellular transport mediated by class V myosins

Informações

Data: 24/03/2014 – 08:30:00

Fim: 24/03/2014 – 17:00:00

Onde: CNPEM – Rua Giuseppe Máximo Scolfaro, 10.000 – Campinas, SÃO PAULO 13280000 Brazil – Telefone: 19 35121267

Detalhes

Abstract: Myosin V (MyoV) motors have been implicated in the intracellular transport of diverse cargoes including vesicles, organelles, RNA-protein complexes, and regulatory proteins. In this seminar, we will present our recent results in this theme including the elucidation of the cargo-binding domain (CBD) structures of the three human MyoV paralogs (Va, Vb, and Vc) and the mapping of cargo-binding sites in CBD-MyoVa. Our findings revealed how subtle structural changes that drive functional differentiation among MyoV paralogs and a novel redox mechanism controlling the CBD dimerization process, which is unique for the MyoVc subclass. Moreover, the cargo- and motor-binding sites were structurally assigned, indicating the conservation of residues involved in the recognition of adaptors for peroxisome transport and providing high resolution insights into motor domain inhibition by CBD. These results contribute to understanding the structural requirements for cargo transport, auto-inhibition, and regulatory mechanisms in myosin V motors.

Speaker: Dr. Mario T. Murakami (LNBio)

24th March 2014 at 8:30am

Room 69 – LNBio
Campus – Centro Nacional de Pesquisa em Energia e Materiais – CNPEM
Rua Giuseppe M. Scolfaro, 10.000 – Guará
Telefone: (19) 3512-1010