Super-Resolution (STED) Microscope

Resource: FINEP (Centros Nacionais Multiusuários 02/2016) convênio 01.17.0024-00

Super-Resolution (STED) Microscope

The Laboratory of Biological Imaging makes available to the external users the Leica TCS SP8 STED 3X inverted super-resolution confocal microscope. This equipment is capable to reach resolution beyond light-diffraction limit for nanoscopy in X, Y and Z (up to 50 nm). It is an open facility to researchers interested in investigating subcellular details in vivo or fixed biological samples through confocal laser scanning mode, tridimensional reconstruction, colocalization, time lapse, FRAP, FRET and spectral separation.

Mode of Operation:

This microscope is fully compatible with regular confocal preparations (i.e. for Leica TCS SP8), including histological section from block embedded in paraffin, O.C.T., resins, agarose, gelatin, etc. Confocal technique can measure thick sample (up to 300 micrometers) after clearing processes. Equipped with stitching module it is able to create panoramic image from multiples regions and positions. In addition to 405nm light source, the microscope uses a pulsed White Light Laser (WLL) which allows using up to 8 channels from 470 to 670 nm, with 1 nm resolution in wavelength. Gating capture is also possible by trigging detector and laser source to deplete background autofluorescence and/or measure fluorescence lifetime.

It is located in the Laboratory of Biological Imaging and includes an TMC anti-vibration table, uninterrupted power supply system, and the equipment is connected to a data storage for remote access.

Useful Information:

Technical specifications: STED depletion laser in 775 nm. Objectives: HC PL Apo CS2 10x/0.40 Dry; HC PL Apo CS2 20x/0.75 Dry; HC PL Apo CS2 63x/1.40 Oil; HC PL Apo CS2 100x/1.40 Oil. Detectors: 2 PMT, 2 Hybrid (gated), 1 TLD. Transmission and DIC capture available.
Access for users: submissions via SAU Online
How to submit proposals: consult the proposal submission guide
Important: contact laboratory staff to discuss sample preparation during the writing of the proposal.
Contact: visit the Team page