Sample Preparation
The user must provide a sample suitable for crystallization taking into consideration the following points:
1 – Purity higher than 95% as assessed by SDS-PAGE or mass spectrometry. The presence of secondary bands in the gel or other contaminants will reduce your success rate.
2- A typical initial protein concentration is 10 mg / ml, but other concentrations are accepted when the Robolab staff is notified.
Standard crystallization kits
“Sparse matrix screens” | ||
Crystal Screen I & II | Sparse matrix by Jancarik & Kim (1991) | In-house |
Wizard I & II | Sparse matrix by Emerald | In-house |
JCSG+ | Sparse matrix by JCSG | In-house |
“Rational screens” | ||
Precipitant Synergy™ | Synergistic combination of mechanistically distinct precipitating agents | In-house |
PACT | For systematic analysis of the effect of pH and anions and cations (by JCSG) | In-house |
“Single-precipitant Grid Screens” | ||
Salt Rx | Salt and pH based crystallization screen | In-house |
“Screens for Optimization” | ||
Additive Screen | library of small molecules that can affect the solubility and crystallization of biological macromolecules | In-house |