The user must provide a sample suitable for crystallization taking into consideration the following points:
1 – Purity higher than 95% as assessed by SDS-PAGE or mass spectrometry. The presence of secondary bands in the gel or other contaminants will reduce your success rate.
2- A typical initial protein concentration is 10 mg / ml, but other concentrations are accepted when the Robolab staff is notified.
Standard crystallization kits
|“Sparse matrix screens”|
|Crystal Screen I & II||Sparse matrix by Jancarik & Kim (1991)||In-house|
|Wizard I & II||Sparse matrix by Emerald||In-house|
|JCSG+||Sparse matrix by JCSG||In-house|
|Precipitant Synergy™||Synergistic combination of mechanistically distinct precipitating agents||In-house|
|PACT||For systematic analysis of the effect of pH and anions and cations (by JCSG)||In-house|
|“Single-precipitant Grid Screens”|
|Salt Rx||Salt and pH based crystallization screen||In-house|
|“Screens for Optimization”|
|Additive Screen||library of small molecules that can affect the solubility and crystallization of biological macromolecules||In-house|