Spectroscopy and Calorimetry

The Spectroscopy and Calorimetry Facility was set up in 2002, as central resource to study proteins biophysics, now it is part of the biophysical target-characterization platform in LNBio. It houses instrumentation to study macromolecules in solution. Among all our the equipments that we have are spectrofotometer, life time fluorimeter, stop flow circular dichroism, isothermal titration calorimetry, differential scanning calorimetry, analytical ultracentrifugation, dynamic light scattering, multimode plate reader, and isoelectric focusing and native electrophoresis. Our scientific mission is to develop and optimize methods to study macromolecular interactions, dynamics and stability.

Aiming structural and biophysical characterization of protein targets in solution, besides protein behavior and characterization measurements, this facility is fully capable of providing detailed information on structure, folding, ligand binding, dissociation constants, association kinetics, stability and oligomeric states. The LEC currently has resources to study oligomeric state of biomolecular assemblies, perform measurements of affinity, stoichiometry, kinetics and thermodynamics of interactions between proteins, DNA and their cognate ligands. Furthermore, the facility executes biophysical characterization capabilities include measurements of molecular weight, shape, and conformation of biological macromolecules.

Our staff provides training, tips for sample preparation and instrument access for all researches. The facility works from Monday to Friday, 8a.m. to 6 p.m., users should submit their purposes on the users portal, in continuous flow. For the equipments that are not in the users portal, users must send one email for rb.mepnc.oibnlnull@cel, requesting and justifying the necessity of the measurement. It is important to prepare the samples and request for the correct number of turns for each experiment to avoid problems in the lab schedule.  The scheduled experiments must start at the beginning of the scheduled period and, in the case of problems, the period must be canceled previously. Do not following the laboratory rules may result in restrictions on the future schedules.

THE USE OF THE FACILITIES MUST BE MENTIONED IN ALL PUBLICATIONS AND THESES GENERATED AS “ACKNOWLEDGMENTS” TO LNBio, AS SET ON THE TERM OF COMMITMENT. THE BIBLIOGRAPHIC REFERENCES OF ANY PUBLICATION AND THESIS GENERATED MUST BE SENT TO THE COORDINATION OF THE LABORATORY.