Informações
Data: 02/09/2013 – 08:30:00
Fim: 02/09/2013 – 17:00:00
Onde: CNPEM – Rua Giuseppe Máximo Scolfaro, 10.000 – Campinas, SÃO PAULO 13280000 Brazil – Telefone: 19 35121267
Detalhes
Abstract: In this seminar, it will be presented the advances in the boundary between cell and molecular biology aiming the achievement of correlative light and electron microscopy images, which reveal high resolution details about the cells and the tissue functions. Each imaging technique provides unique information, and the combination of two or more techniques may contribute to a better understanding of the spatial and temporal patterns of protein expression, function and traffic. For this purpose, specific methods based on immunochemistry and molecular probes may reveal interesting proteins and its location in the cellular environment. Accordingly, the National Center for Microscopy and Imaging Research (NCMIR) at the University of California San Diego (UCSD) has focused on the development of probes that produce singlet oxygen by photo-oxidation (miniSOG – Shu et al. 2011) or enzymatic reaction (APEX – Martell et al., 2012), which allow the localization of proteins by transmission electron microscopy. These techniques were part of a 6-month internship at UCSD. Thus, this seminar will address these techniques for protein subcellular localization and interaction, as well as the technological advances to overcome the resolution limits of light and electronic microscopy in biological and biomedical researches.
Speaker: MSc. Silvio Consonni (LNBio)
2nd September at 8:30 am
Room 69 – LNBio
Campus – Centro Nacional de Pesquisa em Energia e Materiais – CNPEM
Rua Giuseppe M. Scolfaro, 10.000 – Guará
Tel: (19) 3512-1010
