Celigo Imaging Cytometer

The Laboratory of Mammalian primary and cell line/insect cells culture (LCCMI/LCCP) is pleased to offer access to the Celigo Imaging Cytometer from Revvit to external users. This state-of-the-art equipment excels in high-throughput cellular imaging with real-time processing capabilities. The Celigo is designed for rapid and automated cell counting and analysis across a wide range of cell types, including adherent (2D and 3D) and suspension cells, providing detailed insights into cell health, proliferation, and cytotoxicity. This facility is equipped with the necessary infrastructure to support a wide range of cellular imaging applications, making it an excellent resource for researchers aiming to enhance their understanding of cellular dynamics and functions.

Mode of Operation

The Celigo Imaging Cytometer uses bright-field and fluorescent imaging to provide comprehensive cellular analysis. It can perform assays like apoptosis, cell viability, and GFP/RFP expression, among others. This system is adept at handling multiple plate formats (from 6 to 1536 wells) and can generate high-resolution images that facilitate the study of cellular characteristics and behaviors. Equipped with advanced software, it allows for the easy extraction and quantitation of data pertinent to cellular assays.

The Celigo is housed in the LCCMI/LCCP and features a user-friendly interface that enables researchers to quickly set up experiments and modify parameters as needed. Data generated by the Celigo can be directly exported for further analysis, making it an invaluable tool for both exploratory and high-throughput studies.

Useful Information

Technical specifications include bright-field and four fluorescent channels (blue, green, red), making it versatile for various fluorophores. The cytometer’s imaging technology allows for the analysis of cells in situ, preserving the spatial relationships and morphologies of the cells within the culture environment.

Access for users: Access to the Celigo is available through submissions via the SAU Online system.

How to submit proposals: Users are encouraged to consult the proposal submission guide available on our platform.

Important: We advise contacting laboratory staff to discuss specific experimental needs and sample preparation ahead of submitting a proposal.

Contact: For further details and to arrange a consultation, please write to rb.mepncnull@acinumoc.