{"id":58017,"date":"2026-02-02T08:00:43","date_gmt":"2026-02-02T11:00:43","guid":{"rendered":"https:\/\/lnbio.cnpem.br\/?p=58017"},"modified":"2026-01-22T11:38:39","modified_gmt":"2026-01-22T14:38:39","slug":"laboratorio-de-edicao-genomica-leg-do-cnpem-abre-segunda-chamada-para-atendimento-de-usuarios-em-2026","status":"publish","type":"post","link":"https:\/\/lnbio.cnpem.br\/en\/laboratorio-de-edicao-genomica-leg-do-cnpem-abre-segunda-chamada-para-atendimento-de-usuarios-em-2026\/","title":{"rendered":"CNPEM\u2019s Genome Editing Laboratory (LEG) opens second call for user proposals in 2026"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<p style=\"text-align: center;\" data-start=\"248\" data-end=\"394\"><em data-start=\"248\" data-end=\"394\">Facility provides support in experimental design, gene editing strategy development, CRISPR tool production and remote assistance to researchers<\/em><\/p>\n<p style=\"text-align: justify;\" data-start=\"396\" data-end=\"688\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-61437 size-medium\" src=\"https:\/\/i0.wp.com\/cnpem.br\/wp-content\/uploads\/2025\/06\/LEG-Laboratorio-Edicao-Genomica-LNBio.jpg?resize=500%2C281&amp;ssl=1\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" srcset=\"https:\/\/i0.wp.com\/cnpem.br\/wp-content\/uploads\/2025\/06\/LEG-Laboratorio-Edicao-Genomica-LNBio.jpg?resize=500%2C281&amp;ssl=1 500w, https:\/\/i0.wp.com\/cnpem.br\/wp-content\/uploads\/2025\/06\/LEG-Laboratorio-Edicao-Genomica-LNBio.jpg?resize=1030%2C579&amp;ssl=1 1030w, https:\/\/i0.wp.com\/cnpem.br\/wp-content\/uploads\/2025\/06\/LEG-Laboratorio-Edicao-Genomica-LNBio.jpg?resize=768%2C432&amp;ssl=1 768w, https:\/\/i0.wp.com\/cnpem.br\/wp-content\/uploads\/2025\/06\/LEG-Laboratorio-Edicao-Genomica-LNBio.jpg?resize=1536%2C864&amp;ssl=1 1536w, https:\/\/i0.wp.com\/cnpem.br\/wp-content\/uploads\/2025\/06\/LEG-Laboratorio-Edicao-Genomica-LNBio.jpg?w=1920&amp;ssl=1 1920w\" alt=\"\" width=\"500\" height=\"281\" data-recalc-dims=\"1\" \/>The Genome Editing Laboratory (LEG), a facility of the Brazilian Center for Research in Energy and Materials (CNPEM), is launching its second call for user proposals. Proposal submissions will take place from\u00a0<strong data-start=\"605\" data-end=\"640\">February 2 to February 27, 2026<\/strong>, exclusively through the\u00a0<a href=\"https:\/\/sauonline.cnpem.br\/duo\/\" target=\"_blank\" rel=\"noopener\">SAU Online\u00a0<\/a>system.<\/p>\n<p style=\"text-align: justify;\" data-start=\"690\" data-end=\"986\">Operated by the Brazilian Biosciences National Laboratory (LNBio), the facility offers technical and scientific support for the generation of mammalian knockout cells using\u00a0<strong data-start=\"863\" data-end=\"878\">CRISPR\/Cas9<\/strong>\u00a0technology, with an emphasis on strategic experimental planning and the production of genome editing tools.<\/p>\n<p style=\"text-align: justify;\" data-start=\"988\" data-end=\"1202\">The initiative aims to expand national access to gene editing technologies, fostering technological autonomy and accelerating the development of models for biomedical, pharmacological and biotechnological research.<\/p>\n<p style=\"text-align: justify;\" data-start=\"1204\" data-end=\"1245\">Main services offered by LEG include:<\/p>\n<ul style=\"text-align: justify;\" data-start=\"1247\" data-end=\"2097\">\n<li data-start=\"1247\" data-end=\"1541\">\n<p data-start=\"1249\" data-end=\"1541\"><strong data-start=\"1249\" data-end=\"1311\">Experimental design and gene editing strategy development:<\/strong>\u00a0Support in defining the most appropriate approach for CRISPR\/Cas9-mediated gene knockout, including guide RNA selection, validation strategies and analysis of potential off-target effects, limited to up to two genes per proposal;<\/p>\n<\/li>\n<li data-start=\"1542\" data-end=\"1741\">\n<p data-start=\"1544\" data-end=\"1741\"><strong data-start=\"1544\" data-end=\"1575\">Production of CRISPR tools:<\/strong>\u00a0Manufacturing of customized molecular components such as sgRNAs, plasmid vectors and ribonucleoprotein (RNP) complexes, according to the defined experimental design;<\/p>\n<\/li>\n<li data-start=\"1742\" data-end=\"2097\">\n<p data-start=\"1744\" data-end=\"2097\"><strong data-start=\"1744\" data-end=\"1763\">Remote support:<\/strong>\u00a0Remote technical guidance to assist users in applying CRISPR tools in their own laboratories, including recommendations on transfection, clone selection and genotypic validation. Support will be provided in up to two remote meetings, with a maximum duration of two hours each, during the project execution period defined in the call.<\/p>\n<\/li>\n<\/ul>\n<p style=\"text-align: justify;\" data-start=\"2099\" data-end=\"2362\">As part of the strategy to broaden national access to the technology, proposals from Brazil\u2019s\u00a0<strong data-start=\"2193\" data-end=\"2238\">North, Northeast and Central-West regions<\/strong>\u00a0will be prioritized. Selected users may also follow, in person at LNBio, the experiments related to CRISPR tool production.<\/p>\n<h3 style=\"text-align: justify;\" data-start=\"2364\" data-end=\"2388\">Eligibility criteria<\/h3>\n<p style=\"text-align: justify;\" data-start=\"2390\" data-end=\"2442\">Eligible proposals must meet the following criteria:<\/p>\n<ul style=\"text-align: justify;\" data-start=\"2444\" data-end=\"2770\">\n<li data-start=\"2444\" data-end=\"2536\">\n<p data-start=\"2446\" data-end=\"2536\">Projects aimed at constitutive knockout of up to two target genes in mammalian cell lines;<\/p>\n<\/li>\n<li data-start=\"2537\" data-end=\"2612\">\n<p data-start=\"2539\" data-end=\"2612\">Proposals involving conditional knockout strategies will not be accepted;<\/p>\n<\/li>\n<li data-start=\"2613\" data-end=\"2770\">\n<p data-start=\"2615\" data-end=\"2770\">Only one proposal per principal investigator will be considered. In the event of multiple submissions, only the first proposal submitted will be evaluated.<\/p>\n<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\" data-start=\"2772\" data-end=\"2808\">How to submit research proposals<\/h3>\n<p style=\"text-align: justify;\" data-start=\"2810\" data-end=\"2959\">Researchers interested in using the LEG infrastructure must submit their proposals via\u00a0<strong data-start=\"2897\" data-end=\"2911\">SAU Online<\/strong>, a portal managed by CNPEM\u2019s User Office (EdU).<\/p>\n<p style=\"text-align: justify;\" data-start=\"2961\" data-end=\"3125\">LEG calls for proposals are published semiannually. In this\u00a0<strong data-start=\"3021\" data-end=\"3043\">first call of 2026<\/strong>, selected projects may be carried out between\u00a0<strong data-start=\"3090\" data-end=\"3124\">April 1 and September 30, 2026<\/strong>.<\/p>\n<p style=\"text-align: justify;\" data-start=\"3127\" data-end=\"3414\">Before submitting proposals, general questions may be sent to CNPEM\u2019s User Office at\u00a0<strong data-start=\"3212\" data-end=\"3228\"><a class=\"decorated-link cursor-pointer\" rel=\"noopener\" data-start=\"3214\" data-end=\"3226\">edu@cnpem.br<\/a><\/strong>. Technical questions related specifically to the facility should be directed to\u00a0<strong data-start=\"3309\" data-end=\"3331\"><a class=\"decorated-link cursor-pointer\" rel=\"noopener\" data-start=\"3311\" data-end=\"3329\">leg@lnbio.cnpem.br<\/a><\/strong>. For information on financial support, please visit\u00a0<a href=\"https:\/\/cnpem.br\/en\/edu\/financial-support\/\" target=\"_blank\" rel=\"noopener\">CNPEM\u2019s financial support webpage<\/a>.<\/p>\n<h3 style=\"text-align: justify;\" data-start=\"3416\" data-end=\"3435\">Important dates<\/h3>\n<ul style=\"text-align: justify;\" data-start=\"3437\" data-end=\"3598\">\n<li data-start=\"3437\" data-end=\"3485\">\n<p data-start=\"3439\" data-end=\"3485\"><strong data-start=\"3439\" data-end=\"3463\">Proposal submission:<\/strong>\u00a0February 2\u201327, 2026;<\/p>\n<\/li>\n<li data-start=\"3486\" data-end=\"3544\">\n<p data-start=\"3488\" data-end=\"3544\"><strong data-start=\"3488\" data-end=\"3527\">Announcement of selected proposals:<\/strong>\u00a0March 30, 2026;<\/p>\n<\/li>\n<li data-start=\"3545\" data-end=\"3598\">\n<p data-start=\"3547\" data-end=\"3598\"><strong data-start=\"3547\" data-end=\"3569\">Project execution:<\/strong>\u00a0April 1\u2013September 30, 2026.<\/p>\n<\/li>\n<\/ul>\n<div style=\"text-align: justify;\">\n<h2>About LNBio<\/h2>\n<p>The Brazilian Biosciences National Laboratory (LNBio) is dedicated to the study of human health, combining integrative biology with advanced technologies. With competencies in gene editing, microphysiological systems, bioimaging, and tissue engineering, LNBio works to discover molecular targets and develop innovative therapies for illnesses that are of public importance. This broad approach, which includes molecules and living organisms, unravels molecular mechanisms to identify bioactive compounds that are essential to develop new active pharmaceutical ingredients. LNBio concentrates its efforts on demands from the public health system, using state-of-the-art infrastructure and a matrix work model to promote innovation and development at the crossroads between science and health. To integrate health with socioeconomic and environmental factors, LNBio serves as a scientific platform that is available to the government, able to develop advanced technologies that respond to strategic issues. LNBio is part of the Brazilian Center for Research in Energy and Materials (CNPEM) in Campinas, S\u00e3o Paulo, a private, non-profit organization overseen by the Ministry of Science, Technology, and Innovation (MCTI).<\/p>\n<p><a href=\"https:\/\/lnbio.cnpem.br\/en\/\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/lnbio.cnpem.br\/<\/a><\/p>\n<\/div>\n<div style=\"text-align: justify;\">\n<h2>About CNPEM<\/h2>\n<p>The Brazilian Center for Research in Energy and Materials (CNPEM) is home to a state-of-the-art, multi-user and multidisciplinary scientific environment and works on different fronts within the Brazilian National System for Science, Technology and Innovation. A social organization overseen by the Ministry of Science, Technology and Innovation (MCTI), CNPEM is driven by research that impacts the areas of health, energy, renewable materials, and sustainability. It is responsible for Sirius, the largest assembly of scientific equipment constructed in the country, and is currently constructing Project Orion, a laboratory complex for advanced pathogen research. Highly specialized science and engineering teams, sophisticated infrastructure open to the scientific community, strategic lines of investigation, innovative projects involving the productive sector, and training for researchers and students are the pillars of this institution that is unique in Brazil and able to serve as a bridge between knowledge and innovation. CNPEM\u2019s research and development activities are carried out through its four National Laboratories: Synchrotron Light (LNLS), Biosciences (LNBio), Nanotechnology (LNNano), Biorenewables (LNBR), as well as its Technology Unit (DAT) and the Ilum School of Science \u2014 an undergraduate program in Science and Technology supported by the Ministry of Education (MEC).<\/p>\n<p><a href=\"https:\/\/cnpem.br\/en\/\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/cnpem.br\/en\/<\/a><\/p>\n<\/div>\n<div style=\"text-align: justify;\"><\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;] Facility provides support in experimental design, gene editing strategy development, CRISPR tool production and remote assistance to researchers The Genome Editing Laboratory (LEG), a facility of the Brazilian Center for Research in Energy and Materials (CNPEM), is launching its second call for user proposals. Proposal submissions will take place from\u00a0February 2 to February&hellip;<\/p>\n","protected":false},"author":2,"featured_media":57394,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1],"tags":[],"class_list":["post-58017","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticias","category-1","description-off"],"_links":{"self":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/posts\/58017","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/comments?post=58017"}],"version-history":[{"count":0,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/posts\/58017\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/media\/57394"}],"wp:attachment":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/media?parent=58017"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/categories?post=58017"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/tags?post=58017"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}