{"id":57487,"date":"2025-07-28T15:15:26","date_gmt":"2025-07-28T18:15:26","guid":{"rendered":"https:\/\/lnbio.cnpem.br\/?page_id=57487"},"modified":"2026-01-29T09:13:05","modified_gmt":"2026-01-29T12:13:05","slug":"laboratorio-de-vetores-virais","status":"publish","type":"page","link":"https:\/\/lnbio.cnpem.br\/en\/instalacoes-abertas\/laboratorio-de-vetores-virais\/","title":{"rendered":"Viral Vectors Laboratory (LVV)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row full_width=&#8221;stretch_row&#8221; full_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; parallax=&#8221;content-moving&#8221; parallax_image=&#8221;57489&#8243; el_class=&#8221;fundo-escuro&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h1 class=\"interna\"><span style=\"color: #ffffff;\">Viral Vectors Laboratory (LVV)<\/span><\/h1>\n<p>[\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1696512384777{margin-left: -15px !important;border-left-width: -15px !important;padding-left: -15px !important;}&#8221;]\t\t\t<!-- Flexy Breadcrumb -->\r\n\t\t\t<div class=\"fbc fbc-page\">\r\n\r\n\t\t\t\t<!-- Breadcrumb wrapper -->\r\n\t\t\t\t<div class=\"fbc-wrap\">\r\n\r\n\t\t\t\t\t<!-- Ordered list-->\r\n\t\t\t\t\t<ol class=\"fbc-items\" itemscope itemtype=\"https:\/\/schema.org\/BreadcrumbList\">\r\n\t\t\t\t\t\t            <li itemprop=\"itemListElement\" itemscope itemtype=\"https:\/\/schema.org\/ListItem\">\r\n                <span itemprop=\"name\">\r\n                    <!-- Home Link -->\r\n                    <a itemprop=\"item\" href=\"https:\/\/lnbio.cnpem.br\">\r\n                    \r\n                        Home                    <\/a>\r\n                <\/span>\r\n                <meta itemprop=\"position\" content=\"1\" \/><!-- Meta Position-->\r\n             <\/li><li><span class=\"fbc-separator\">\/<\/span><\/li><li class=\"active\" itemprop=\"itemListElement\" itemscope itemtype=\"https:\/\/schema.org\/ListItem\"><span itemprop=\"name\" title=\"Viral Vectors Laboratory (LVV)\">Viral Vectors Laboratory (LVV)<\/span><meta itemprop=\"position\" content=\"2\" \/><\/li>\t\t\t\t\t<\/ol>\r\n\t\t\t\t\t<div class=\"clearfix\"><\/div>\r\n\t\t\t\t<\/div>\r\n\t\t\t<\/div>\r\n\t\t\t[\/vc_column_text][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; gap=&#8221;5&#8243; css=&#8221;.vc_custom_1710876484752{padding-top: 4rem !important;padding-bottom: 4rem !important;background-image: url(https:\/\/lnbio.cnpem.br\/wp-content\/uploads\/2024\/03\/grafismo-organico-lnbio-cinza.png?id=35463) !important;background-position: center !important;background-repeat: no-repeat !important;background-size: cover !important;}&#8221;][vc_column][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3 style=\"text-align: left;\">About the facility<\/h3>\n<p>[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<\/p>\n<p style=\"text-align: justify;\">The Viral Vectors Laboratory (LVV) specializes in the production of recombinant viruses for research and biotechnology applications. The facility operates in a level 2 biosafety environment and is accredited by the National Technical Commission on Biosafety (CTNBio). LVV supports the production of non-replicating recombinant virus preparations, including murine retroviral vectors and human lentiviral vectors derived from HIV-1.<\/p>\n<p style=\"text-align: justify;\">These systems are widely used for gene expression, silencing by RNA interference and generation of modified cell lineages. The facility contributes to the advancement of research aimed at understanding intracellular mechanisms and the development of gene and cell therapies applied to human health.<\/p>\n<p>[\/vc_column_text][vc_empty_space][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3>How to submit research proposals<\/h3>\n<p>[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<\/p>\n<div class=\"vc_row wpb_row vc_inner vc_row-fluid\">\n<div class=\"wpb_column vc_column_container vc_col-sm-12\">\n<div class=\"vc_column-inner\">\n<div class=\"wpb_wrapper\">\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p style=\"text-align: justify;\">Proposals must be submitted through SAU Online. In case of any doubt during the proposal submission, contact the CNPEM Users Office by email at <a href=\"mailto:edu@cnpem.br\">edu@cnpem.br<\/a> or speak to those responsible for the facility by writing to <a href=\"mailto:lvv@lnbio.cnpem.br\">lvv@lnbio.cnpem.br<\/a>.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><div id=\"calendario_modal_69e4f8b70a20b_wrap\" class=\"calendario-modal-wrapper\"><a href=\"\" class=\"botao-base retangular\" target=\"_blank\" >FACILITIES CALENDAR<\/a><\/div><div id=\"calendario_modal_69e4f8b70a20b\" class=\"calendario-modal\" role=\"dialog\" aria-hidden=\"true\">  <div class=\"calendario-modal-content\">    <button class=\"calendario-fechar\" aria-label=\"Close\">&times;<\/button>    <div class=\"calendario-modal-body\">    <div style=\"overflow-x: auto;\">\n        <table style=\"border-collapse: collapse; width: 100%;\">\n            <tbody>\n                <tr>\n                    <td style=\"width: 33.3333%; text-align: center;\"><strong>OPEN FACILITY<\/strong><\/td>\n                    <td style=\"width: 33.3333%; text-align: center;\"><strong>SUBMISSION PERIOD<\/strong><\/td>\n                    <td style=\"width: 33.3333%; text-align: center;\"><strong>EXECUTION PERIOD<\/strong><\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Mass Spectrometry Laboratory (MAS)<\/td>\n                    <td style=\"text-align: center;\">01\/08\/2025 a 31\/08\/2025<\/td>\n                    <td style=\"text-align: center;\">January to December 2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Nuclear Magnetic Resonance Laboratory (RMN)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 30\/11\/2026<\/td>\n                    <td style=\"text-align: center;\">19\/01\/2026 a 15\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Automatic Protein Crystallization Laboratory (ROBOLAB)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 04\/12\/2026<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 11\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Spectroscopy and Calorimetry Laboratory (LEC)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 04\/12\/2026<\/td>\n                    <td style=\"text-align: center;\">06\/01\/2026 a 11\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Biomolecule Synthesis Laboratory (LSB)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 04\/12\/2026<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 11\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Biological Imaging Laboratory (LIB)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 07\/12\/2026<\/td>\n                    <td style=\"text-align: center;\">12\/01\/2026 a 15\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Protein Purification Laboratory (LPP)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 04\/12\/2026<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 11\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Bioassays Laboratory (LBE)<\/td>\n                    <td style=\"text-align: center;\">05\/01\/2026 a 07\/12\/2026<\/td>\n                    <td style=\"text-align: center;\">06\/01\/2026 a 17\/12\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Gene Editing Laboratory (LEG)<\/td>\n                    <td style=\"text-align: center;\">02\/02\/2026 a 27\/02\/2026<\/td>\n                    <td style=\"text-align: center;\">01\/04\/2026 a 30\/09\/2026<\/td>\n                <\/tr>\n\n                <tr>\n                    <td>Viral Vectors Laboratory (LVV)<\/td>\n                    <td style=\"text-align: center;\">03\/01\/2026 a 31\/05\/2026<\/td>\n                    <td style=\"text-align: center;\">01\/02\/2026 a 30\/06\/2026<\/td>\n                <\/tr>\n\n            <\/tbody>\n        <\/table>\n    <\/div>\n        <\/div>  <\/div><\/div><style>\n    .calendario-modal{display:none;position:fixed;z-index:99999;left:0;top:0;width:100%;height:100%;overflow:auto;background-color:rgba(0,0,0,0.6);padding:40px 10px;box-sizing:border-box}\n    .calendario-modal-content{background:#fff;margin:auto;border-radius:8px;padding:18px;max-width:980px;box-shadow:0 10px 30px rgba(0,0,0,.3);position:relative}\n    .calendario-fechar{position:absolute;right:18px;top:14px;border:none;background:transparent;font-size:28px;cursor:pointer}\n    .calendario-modal-body{margin-top:10px}\n    @media (max-width:600px){.calendario-modal{padding-top:20px}.calendario-modal-content{width:100%;padding:12px}}\n\n    \/* ===== TABELA ===== *\/\n    .calendario-modal-content table{width:100%;border-collapse:collapse;font-family:inherit;font-size:1rem;font-weight:normal}\n    .calendario-modal-content td,.calendario-modal-content th{border:1px solid #ddd;padding:8px;text-align:center;vertical-align:middle;font-weight:normal}\n    .calendario-modal-content tr:first-child td,.calendario-modal-content thead tr th{background:#f5f5f5;font-weight:bold}\n    <\/style><script>\n    (function(){\n        var uid = \"calendario_modal_69e4f8b70a20b\";\n        var wrap = document.getElementById(uid + \"_wrap\");\n        var modal = document.getElementById(uid);\n\n        function openModal(){\n            modal.style.display = \"block\";\n            modal.setAttribute(\"aria-hidden\",\"false\");\n            document.body.style.overflow = \"hidden\";\n        }\n        function closeModal(){\n            modal.style.display = \"none\";\n            modal.setAttribute(\"aria-hidden\",\"true\");\n            document.body.style.overflow = \"\";\n        }\n\n        if(wrap){\n            var trigger = wrap.querySelector(\"a, button, [role=button], input[type=button], .botao-retangular\");\n            if(trigger){\n                trigger.addEventListener(\"click\", function(e){\n                    e.preventDefault();\n                    e.stopPropagation();\n                    openModal();\n                });\n            }\n        }\n\n        document.addEventListener(\"click\", function(e){\n            if(e.target.closest(\"#\" + uid + \" .calendario-fechar\") || e.target.id === uid){\n                closeModal();\n            }\n        });\n\n        document.addEventListener(\"keydown\", function(e){\n            if((e.key === \"Escape\" || e.keyCode === 27) && modal.style.display === \"block\"){\n                closeModal();\n            }\n        });\n    })();\n    <\/script><\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><a href=\"https:\/\/sauonline.cnpem.br\/duo\/\" class=\"botao-base retangular\" target=\"_blank\" >SUBMIT YOUR PROPOSAL<\/a><\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text css=&#8221;&#8221;]<\/p>\n<h3 style=\"text-align: center;\"><a href=\"https:\/\/lnbio.cnpem.br\/wp-content\/uploads\/2025\/07\/FAQ-LVV-LNBio.pdf\" class=\"botao-base retangular\" target=\"_blank\" >SEE THE FAQ<\/a><\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_cta h2=&#8221;Equipment&#8221; h2_font_container=&#8221;tag:h3|text_align:left&#8221; h2_use_theme_fonts=&#8221;yes&#8221; shape=&#8221;square&#8221; add_icon=&#8221;left&#8221; i_icon_fontawesome=&#8221;fas fa-microscope&#8221; i_size=&#8221;sm&#8221; css=&#8221;&#8221; use_custom_fonts_h2=&#8221;true&#8221;]The LVV is installed in a culture room dedicated to the production of viral preparations, with the following equipment:<\/p>\n<ul>\n<li>Class II safety cabinets;<\/li>\n<li>CO\u2082 incubators for cell lineage maintenance;<\/li>\n<li>Inverted microscope;<\/li>\n<li>Preparative centrifuges;<\/li>\n<li>Ultracentrifuge for particle concentration;<\/li>\n<li>\u201380\u00ba C Biofreezer for production preservation;<\/li>\n<li>Novocyte 2000 flow cytometer.<\/li>\n<\/ul>\n<p>[\/vc_cta][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_cta h2=&#8221;Main competencies and techniques&#8221; h2_font_container=&#8221;tag:h3|text_align:left&#8221; h2_use_theme_fonts=&#8221;yes&#8221; shape=&#8221;square&#8221; add_icon=&#8221;left&#8221; i_icon_fontawesome=&#8221;fas fa-network-wired&#8221; i_size=&#8221;sm&#8221; css=&#8221;&#8221; use_custom_fonts_h2=&#8221;true&#8221;]<\/p>\n<ul>\n<li>Production of murine retroviral vectors with ecotropic, amphotropic, 10A1, RD114 or VSV-G envelopes;<\/li>\n<li>Production of lentiviral vectors derived from human HIV-1 with the same envelopes;<\/li>\n<li>Customized productions with envelopes provided by the applicant, subject to technical feasibility.<\/li>\n<\/ul>\n<p>[\/vc_cta][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1710521711366{background-color: #eff2f5 !important;background-position: center !important;background-repeat: no-repeat !important;background-size: cover !important;}&#8221;][vc_column][vc_empty_space height=&#8221;16px&#8221;][vc_custom_heading text=&#8221;meet our team&#8221; font_container=&#8221;tag:h3|text_align:left&#8221; use_theme_fonts=&#8221;yes&#8221; css=&#8221;&#8221;][vc_row_inner][vc_column_inner width=&#8221;1\/4&#8243;]<div class=\"ult-team-member-wrap ult-style-1  \"><div class=\"ult-team-member-image\" style=\"\"> <img decoding=\"async\" class=\"ult-team-grayscale\" src=\"\" alt=\"\"  style=\"\"><span class=\"ult-team-member-image-overlay \"  ><\/span><\/div><div class=\"ult-team-member-bio-wrap style-1 ultimate-heading69e4f8b70a4a0\" style=\"text-align:center;; \"><div class=\"ult-team-member-name-wrap\"><h2 class=\"ult-team-member-name ult-responsive\"  data-ultimate-target='.ult-team-member-bio-wrap.ultimate-heading69e4f8b70a4a0 .ult-team-member-name'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"\">Marcio C. Bajgelman<\/h2><div class=\"ult-team-member-position ult-responsive\"  data-ultimate-target='.ult-team-member-bio-wrap.ultimate-heading69e4f8b70a4a0 .ult-team-member-position'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"\">Coordinator<\/div><div class=\"ult-team-member-description ult-responsive\"  data-ultimate-target='.ult-team-member-bio-wrap.ultimate-heading69e4f8b70a4a0 .ult-team-member-description'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"\"><p><a href=\"mailto:lvv@lnbio.cnpem.br\">lvv@lnbio.cnpem.br<\/a><\/p><\/div><div style=\"margin-bottom:15px\"><\/div><\/div><\/div><\/div>[\/vc_column_inner][vc_column_inner width=&#8221;1\/4&#8243;]<div class=\"ult-team-member-wrap ult-style-1  \"><div class=\"ult-team-member-image\" style=\"\"> <img decoding=\"async\" class=\"ult-team-grayscale\" src=\"\" alt=\"\"  style=\"\"><span class=\"ult-team-member-image-overlay \"  ><\/span><\/div><div class=\"ult-team-member-bio-wrap style-1 ultimate-heading69e4f8b70a50d\" style=\"text-align:center;; \"><div class=\"ult-team-member-name-wrap\"><h2 class=\"ult-team-member-name ult-responsive\"  data-ultimate-target='.ult-team-member-bio-wrap.ultimate-heading69e4f8b70a50d .ult-team-member-name'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"\">Daniela S. Mizobuti<\/h2><div class=\"ult-team-member-position ult-responsive\"  data-ultimate-target='.ult-team-member-bio-wrap.ultimate-heading69e4f8b70a50d .ult-team-member-position'  data-responsive-json-new='{\"font-size\":\"\",\"line-height\":\"\"}'  style=\"\">Specialist<\/div><div style=\"margin-bottom:15px\"><\/div><\/div><\/div><\/div>[\/vc_column_inner][vc_column_inner width=&#8221;1\/4&#8243;][\/vc_column_inner][vc_column_inner width=&#8221;1\/4&#8243;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1696434766725{background-color: #ffffff !important;background-position: center !important;background-repeat: no-repeat !important;background-size: cover !important;}&#8221;][vc_column][vc_empty_space height=&#8221;40&#8243;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row full_width=&#8221;stretch_row&#8221; full_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; parallax=&#8221;content-moving&#8221; parallax_image=&#8221;57489&#8243; el_class=&#8221;fundo-escuro&#8221;][vc_column][vc_column_text css=&#8221;&#8221;] Viral Vectors Laboratory (LVV) [\/vc_column_text][vc_column_text css=&#8221;.vc_custom_1696512384777{margin-left: -15px !important;border-left-width: -15px !important;padding-left: -15px !important;}&#8221;][\/vc_column_text][\/vc_column][\/vc_row][vc_row full_width=&#8221;stretch_row&#8221; gap=&#8221;5&#8243; css=&#8221;.vc_custom_1710876484752{padding-top: 4rem !important;padding-bottom: 4rem !important;background-image: url(https:\/\/lnbio.cnpem.br\/wp-content\/uploads\/2024\/03\/grafismo-organico-lnbio-cinza.png?id=35463) !important;background-position: center !important;background-repeat: no-repeat !important;background-size: cover !important;}&#8221;][vc_column][vc_column_text css=&#8221;&#8221;] About the facility [\/vc_column_text][vc_column_text css=&#8221;&#8221;] The Viral Vectors Laboratory (LVV) specializes in the production of recombinant viruses for research&hellip;<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":52223,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[124],"tags":[],"class_list":["post-57487","page","type-page","status-publish","hentry","category-open-facilities","description-off"],"_links":{"self":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/57487","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/comments?post=57487"}],"version-history":[{"count":0,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/57487\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/pages\/52223"}],"wp:attachment":[{"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/media?parent=57487"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/categories?post=57487"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lnbio.cnpem.br\/en\/wp-json\/wp\/v2\/tags?post=57487"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}