Tag Archive

  • DNA Microarray

    DNA microarray facility (LMA) performs analysis of gene expression to identify genes involved in functional and pathogenic processes. In this facility it is possible to carry out analysis of genome wide gene expression and the identification of single nucleotide polymorphisms.

    The LMA platform is from Affymetrix™ and comprises: a hybridization oven, two fluidic stations, a scanner and a workstation with two Zeon processors and operating software also from Affymetrix.

  • Crystallization of Macromolecules

    ROBOLAB is the most highly equipped high-throughput laboratory for the crystallization of macromolecules and their complexes, which is open to the scientific community in Latin America. Its mission is to provide state-of-the-art technologies and equipment for the crystallization of macromolecules increasing the chance of success in obtaining crystals.

     

  • Bioassays

    The Bioassay Laboratory (LBE) is focused on assay development, high throughput screening and high content cellular analysis. The LBE operates the first Cell::Explorer platform (PerkinElmer) in  South America.

    The Cell::Explorer is equipped with a microplate fluorescence reader (ENVISION), a confocal microscope (Operetta), a 384 pippeting unit (JANUS-MDT), a microplate washer (Biotek), cell Incubator (Liconic), and stacker for 45 microplates. All plates are handled by a robotic arm inside an air filtered environment.

    At the moment the LBE team is working to validate HTS bioassays for reductases and kinases targets. The LBE compound collection contains samples from the NIH Clinical Collection and DIVERSET compound library (Chembridge).

    The LBE will soon open a new agenda  for external users interested in running HTS campaigns against reductases or kinases targets.

    For additional information, please contact one of the LBE coordinators.

  • Bioinformatics

    The Laboratory of Bioinformatics (LBI) was created to meet the needs of storing and analyzing the data sets of biological data whose complexity requires the use of statistical, mathematical and computational methods. The LBI, is  an unusual model in Brazil, presenting scientific cores that meet both the demand for research in bioinformatics applied to structural biology as well as in bioinformatics applied to genomics, transcriptomics, metabolomics and proteomics.

    The main focus of  the  bioinformatics structural core is  the application of computational methods for the study of intermolecular interactions in biological and pathological models. Among the protocols in place, we highlight the computational platform that allows the analysis of the binding potential of small molecules to proteins, coupled with new methods of computational analysis devoted to obtaining the structural features of  cavities in proteins, with a strong involvement in studies for the development of new therapeutic agents . Also, in the field of functional characterization of proteins, LBI works with a number of protocols in silico structural determination and molecular dynamics simulation, which allows researchers to accurately predict the experimental molecular features to be explored in bench experiments.

    The bioinformatics core is focused on experimental design and data analysis related to genomes, transcriptomes, proteomes conducts applied research on human health and agricultural solutions. This core develops protocols for computational analysis of metagenomics, the analysis of differential gene expression in microarray data and next-generation sequencing, and analysis of protein-protein interaction generated in proteomics experiments and two-hybrid.