Available Services and Technologies

The experiments available are focused on the determination of protein structures and the dynamic properties of proteins, as well as on the characterization of protein-ligand interactions and metabolic profile of biological samples.

Homonuclear, heteronuclear and two-dimensional (2D) or three-dimensional (3D) NMR techniques are available, including:

  • 3D pulse sequences observing or decoupling 1H, 13C and 15N nuclei
    • determination of amino acid connectivity in proteins;
    • side chain attribution;
    • extraction of distance restraints based on Nuclear Overhauser Effect (NOE).
  • 2D pulse sequence for structural elucidation of organic compounds
    • COSY (Correlation Spectroscopy), HSQC (Heteronuclear Single Quantum Coherence Spectroscopy), HMBC (Heteronuclear Multiple Bond Connectivity), TOCSY (Total Correlation Spectroscopy), NOESY (Nuclear Overhauser Effect Spectroscopy), ROESY (Rotating Frame Nuclear Overhauser Effect Spectroscopy).
  • 1D pulse sequence to provide information about protein binding complexes.
    • 1H-NMR STD (Saturation Transfer Difference) and WaterLOGSY (Water Ligand Observation with Gradient Spectroscopy)
  • 1D pulse sequence for the construction of metabolic profiles.
    • several protocols were developed based on blood serum, urine, cells, tissues, etc.