Sample Preparation

The user must provide a sample suitable for crystallization taking into consideration the following points:

1 – Purity higher than 95% as assessed by SDS-PAGE or mass spectrometry. The presence of secondary bands in the gel or other contaminants will reduce your success rate.

2- A typical initial protein concentration is 10 mg / ml, but other concentrations are accepted when the Robolab staff is notified.

 

 Standard crystallization kits

“Sparse matrix screens”

Crystal Screen I & II

Sparse matrix by Jancarik & Kim (1991)

In-house

Wizard I & II

Sparse matrix by Emerald

In-house

JCSG+

Sparse matrix by JCSG

In-house

“Rational screens”

Precipitant Synergy™

Synergistic combination of mechanistically distinct precipitating agents

In-house

PACT

For systematic analysis of the effect of pH and anions and cations (by JCSG)

In-house

“Single-precipitant Grid Screens”

Salt Rx

Salt and pH based crystallization screen

In-house

“Screens for Optimization”

Additive Screen

library of small molecules that can affect the solubility and crystallization of biological macromolecules

In-house