MX 2 (5-15 keV, 0.8-2.5 Å)

MX2 – Macromolecular Crystallography Beamline 2 was the first dedicated protein crystallography wiggler beamline in Latin America suitable both for native and anomalous data collection. Brazilian and Latin American researchers benefit from the MX2 experimental capabilities to unveil the structure of proteins and macromolecular assemblies and give  insights into the action mechanism  of biological molecules.

First MX2 Crystallographic results. (a) Crystal structure of HEWL solved using the sulfur signal. (b) Electron density map calculated from SAD experimental phases of a Leptospira interrogans putative lipoprotein crystal (PDB code 3BWS).

MX2 has been in operation since 2007 and offers wide tunability between 5 and 15 keV and a measured flux density of 4.8 x 1011 photons s-1 mm-2 (100 mA)-1 at the sample position at 8.7 eV. The beamline is equipped with a 3 x 3 MarMosaic225 CCD detector and is controlled by a customized version of the Blu-Ice software.

The optical layout consists of a parabolic cylinder collimating mirror that sends collimated light onto the flat Si(111) monochromator crystals (DCM). The monochromatic light is refocused by a toroidal bendable mirror.

Recently a G-rob system (NatXray) has been implemented at the MX2 beamline, allowing automated mounting of cryo-protected crystals as well as crystallization plates.

G-rob system (NatXray)